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Nuclear magnetic resonance spectroscopy to determine the micellar cholesterol in human bile
Author(s) -
Ellul J.P.M.,
Murphy G.M.,
Parkes H.G.,
Slapa R.Z.,
Dowling R.H.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80158-d
Subject(s) - cholesterol , proton magnetic resonance , nuclear magnetic resonance spectroscopy , chemistry , nuclear magnetic resonance , spectroscopy , micellar solutions , proton , bile acid , resonance (particle physics) , micelle , biochemistry , stereochemistry , organic chemistry , physics , aqueous solution , atomic physics , quantum mechanics
The cholesterol of gallstones comes from the vesicular rather than the micellar phase of bile. Progress in this field has been limited because conventional analytical methods disturb the distribution of cholesterol between the two phases. The resonance of the cholesterol C 6 proton occurs at a chemical shift of 5.4 ppm, to be shown by 2D NMR to be specific for biliary cholesterol, and arises only from the micellar mode. Thus integration of C 6 proton resonance peak area provides a direct non‐invasive determination of the cholesterol distribution in human bile.