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Detection of elsamicin‐DNA binding specificity by restriction enzyme cleavage
Author(s) -
Párraga Antonio,
Portugal José
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80157-c
Subject(s) - restriction enzyme , cleavage (geology) , chemistry , dna , enzyme , biochemistry , microbiology and biotechnology , biology , paleontology , fracture (geology)
The sequence specificity of elsamicin A, an anti‐tumour antibiotic, binding to DNA was elucidated considering the inhibition of the rate of digestion of linearised pBR322 DNA by An II, Cla I, Eco RI, Hind III and Nru I restriction enzymes. Elsamicin A inhibits the rate of digestion by Nru I (recognition sequence TCG/CGA) to a greater extent than it does for the other enzymes thus evidencing the sequence‐selective binding of elsamicin to CGC regions in DNA. Our results also show the important role of the neighbouring sequences in the elsamicin A‐DNA interactions and their effects on the cleavage by restriction enzymes.