Premium
Analysis of a Nicotiana plumbaginifolia cDNA encoding a novel small GTP‐binding protein
Author(s) -
Terryn N.,
Anuntalabhochai S.,
Van Montagu M.,
Inzé D.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80133-2
Subject(s) - complementary dna , gtp' , biology , biochemistry , nicotiana , gtp binding protein regulators , binding protein , amino acid , g protein , arabidopsis , genetics , microbiology and biotechnology , gene , enzyme , signal transduction , solanaceae , mutant
Small GTP‐binding proteins belonging to the Ras superfamily have been found in evolutionarily divergent organisms. Here, we report the isolation and analysis of a cDNA encoding a putative small GTP‐binding protein, designated Rhn1, from the plant, Nicotiana plumbaginifolla . The 21.8‐kDa protein has 60% amino acid similarity with the mammalian Rab5 proteins. The Rhn1 protein is encoded by a small multigene family. Northern analysis shows the highest steady‐state mRNA levels to be in roots and flowers. Furthermore, the Rhnl protein has 80% amino acid similarity with an Arabidopsis small GTP‐binding protein, designated Rha1.