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Growth factor‐stimulated trans plasma membrane electron transport in HL‐60 cells
Author(s) -
Navas Placido,
Alcain Francisco J.,
Burón Isabel,
Rodríquez-Aguilera Juan-Carlos,
Villalba Jose M.,
Morré Dorothy M.,
Morré D.James
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80119-2
Subject(s) - electron transport chain , biophysics , chemistry , membrane , electron acceptor , electron , plasma , electron transfer , biochemistry , photochemistry , biology , physics , quantum mechanics
Electron flow across the plasma membrane of living cells and its rapid modulation by growth factors has been measured continuously through a simple assay procedure whereby the transported electrons are captured by ascorbate free radical to slow the rate of chemical oxidation of ascorbate. The assay provides a direct demonstration of electron transport to an external electron acceptor that is both physiological and impermeant. The reduction of external ascorbate free radical is stimulated by the growth factors, EGF and transform, and is inhibited by wheat germn agglutinin. The results demonstrate, under physiological conditions, the operation of a growth factor‐ and lecitin‐responsive electron transport system at the cell surface using a cultured human cell line.