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Quantitation of a mitochondrial DNA deletion in Parkinson's disease
Author(s) -
Mann Vincent M.,
Cooper J.Mark,
Schapira Anthony H.V.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80118-z
Subject(s) - mitochondrial dna , substantia nigra , pathogenesis , parkinson's disease , polymerase chain reaction , mitochondrial respiratory chain , biology , microbiology and biotechnology , mitochondrion , dna , genetics , respiratory chain , disease , gene , medicine , pathology , immunology
A 5 kilobase deletion in mitochondrial DNA (mtDNA) has been reported to be responsible for the specific complex I deficiency in the substantia nigra (SN) of the Parkinson's disease (PD) brain. We have studied mitochondrial respiratory chain function in the SN from control and PD subjects, and analysed mtDNA, extracted from the same tissues, by Southern biot and the polymerase chain reaction (PCR). Quantitation of the levels of the deletion indicate that it does not contribute to the pathogenesis of PD nor to a complex I deficiency but seems likely to be an age‐related observation.