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Biological activity of recombinant Ricinus communis agglutinin A chain produced in Escherichia coli
Author(s) -
O'Hare Mary,
Roberts Lynne M.,
Lord J.Michael
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80116-x
Subject(s) - ricin , ricinus , recombinant dna , agglutinin , escherichia coli , microbiology and biotechnology , biochemistry , biology , plasmid , lectin , chemistry , dna , gene , toxin
DNA encoding Ricinus communis agglutinin A chain was listed into the E. coli expression vector pDS S/3. Induced E. coli 71.18 cells which had been transformed with this plasmid express Ricinus communis agglutinin A chain in a soluble and biologically active form. Recombinant Ricinus communis agglutinin A chain had ribosomal RNA N ‐glycosidase activity and was approximately 10‐fold less active than ricin A chain in a cell‐free protein synthesis inhibition assay.