Premium
Inhibitory effect of oryzacystatins and a truncation mutant on the replication of poliovirus in infected Vero cells
Author(s) -
Kondo Hiroto,
Ijiri Sumiko,
Abe Keiko,
Maeda Hiroshi,
Arai Soichi
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80097-z
Subject(s) - vero cell , poliovirus , picornavirus , cysteine , viral replication , mutant , biology , in vitro , proteinase k , microbiology and biotechnology , virology , biochemistry , chemistry , virus , enzyme , rna , gene
Poliovirus, a picornavirus family member, requires the processing of its poly‐protein by its own cysteine proteinase for replication. Oryzacystatin‐I and oryzacystatin‐II, proteinaceous cysteine proteinase inhibitors (cystatins) of rice seed origin, were found to inhibit the replication of poliovirus effectively in infected Vero cells in vitro. Truncated oryzacystatin‐I, which lacks the NH 2 ‐terminal 25 amino acid residues of the intact protein, is an even more effective inhibitor, eliciting its effect at concentrations of less than 0.23 nmol/ml. The low molecular weight cysteine proteinase inhibitors, E‐64, E‐64C and loxistatin, showed no anti‐viral effect at any concentration investigated.