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Molecular cloning of an essential yeast gene encoding a proteasomal subunit
Author(s) -
Georgatsou Elena,
Georgakopoulos Tassos,
Thireos George
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80095-x
Subject(s) - protein subunit , cloning (programming) , saccharomyces cerevisiae , gene , yeast , proteasome , biology , molecular cloning , microbiology and biotechnology , genetics , biochemistry , peptide sequence , computer science , programming language
We present the cloning and sequence of a Saccharomyces cerevisiae gene , PUP2 , which encodes for a proteasomal subunit. The PUP2 protein is similar to other proteasomal components from yeast, as well as from Drosophila and rat. Although not‐properly‐folded proteins have been implicated to constitute substrates of proteasomes, we show that the accumulation of such proteins does not induce expression of the PUP2 gene. Finally, gene disruption experiments demonstrate that PUP2 belongs to the class of yeast proteasomal subunits that are essential for cell viability.