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Changes in the expression of mRNAs for myogenic factors and other muscle‐specific proteins in experimental autoimmune myasthenia gravis
Author(s) -
Asher Orna,
Fuchs Sara,
Zuk Dorit,
Rapaport Debora,
Buonanno Andres
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80089-y
Subject(s) - myasthenia gravis , immunology , autoimmune disease , biology , antibody
The regulation of genes for acetylcholine receptor (AChR), myogenic factors and other muscle‐specific proteins has been analyzed in experimental autoimmune myasthenia gravis (EAMG) and following denervation. The levels of the transcripts for the myogenic factors, MyoDl, myogenin and MRF4, were measured using Northern blot analysis. Myogenin and MRF4 transcript levels were observed to be 3.1 ‐ and 2.6‐fold higher in muscle of rats with EAMG than in controls, respectively. MyoDl levels, however, remained unchanged. The increases in AChR, myogenin and MRF4 mRNAs were one order of magnitude higher in 2‐week denervated muscle than in the myasthenic muscle. The levels of muscle creatine kinase (MCK), α‐actin and muscle dystrophin transcripts were also analyzed. Dystrophin levels were found to be 1.7‐ and 4.7‐fold higher in EAMG and denervated muscle, respectively, than in controls; in contrast, MCK and α‐actin levels remained unchanged.