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Conservation of binding site specificity of three yeast DNA binding proteins
Author(s) -
Pretorius G.H.J.,
Muller H.E.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80057-n
Subject(s) - saccharomyces cerevisiae , yeast , oligonucleotide , binding site , biochemistry , dna , affinities , biology , mutant , plasma protein binding , dna binding site , chemistry , gene , gene expression , promoter
Sequence specific binding of protein extracts from 13 different yeast species to three oligonucleotide probes and two points mutants derived from Saccharomyces cerevisiae DNA binding proteins were tested using mobility shift assays. The probes were high affinity binding sites for GRF1/RAP1/ ABF1 and CP1/CPF1. Most yeasts in the genus Saccharomyces showed specific binding to all three probes and also displayed similar sequence requirements when challenged by molar excesses of mutant probes. The affinities for the probes varied amongst the other yeasts tested, but in general, CPF1 binding activity was the most widespread, while the other two were more limited.