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Immunoreactive thyroliberin (TRH) precursor forms in human hypothalamus and anterior pituitary tissues
Author(s) -
Croissandeau G.,
Pagésy P.,
Grouselle D.,
Le Dafniet M.,
Peillon F.,
Li J.Y.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80054-k
Subject(s) - anterior pituitary , hypothalamus , antiserum , medicine , endocrinology , thyrotropin releasing hormone , pituitary gland , chemistry , biology , hormone , antibody , immunology
Immunoreactive (IR) proTRH forms were characterized in human hypothalamic tissue with two antisera raised against a hepta‐ and a decapeptide containing the TRH progenitor sequence (‐Gln‐His‐Pro‐Gly‐). A similar study was performed in human normal and adenomatous anterior pituitaries, tissues in which TRH synthesis has been previously suggested. IR‐proTRH was found in all the samples ranging from 42–775 fmol/mg proteins. Size exclusion chromatography identified a major 25–35 kDa form and a minor 4–8 kDa form. The existence of the major form was confirmed by immunoblotting. The results suggest that both human hypothalamic and normal or adenomatous anterior pituitary tissues synthesize similar IR‐proTRH forms.