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ASP‐56, a new actin sequestering protein from pig platelets with homology to CAP, an adenylate cyclase‐associated protein from yeast
Author(s) -
Gieselmann Ralph,
Mann Karlheinz
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80043-g
Subject(s) - adenylate kinase , cyclase , chemistry , homology (biology) , yeast , actin , platelet , biochemistry , microbiology and biotechnology , biology , enzyme , gene , immunology
A new 56 kDa actin‐binding protein (ASP‐56) was isolated from pig platelet lysate. In falling ball viscosimetry it caused a reduction in viscosity that could be attributed to a decrease in the concentration of polymeric actin. Fluorescence measurements with NBD‐labelled actin showed reduction of polymeric actin, too. These results could be explained by sequestering of actin in a non‐polymerizable 1:1 ASP‐56/actin complex. Sequencing of about 20 tryptic peptides of ASP‐56 and comparison with known sequences revealed about 60% homology to the adenylate cyclase‐associated protein (CAP) from yeast.