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rol A modulates the sensitivity to auxin of the proton translocation catalyzed by the plasma membrane H + ‐ATPase in transformed tobacco
Author(s) -
Vansuyt Gérard,
Vilaine Françoise,
Tepfer Mark,
Rossignol Michel
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80028-f
Subject(s) - auxin , chromosomal translocation , stimulation , atpase , vesicle , membrane , chemistry , microbiology and biotechnology , biology , biochemistry , gene , biophysics , enzyme , endocrinology
In order to investigate the effect of the rol A gene product on the plasma membrane response to auxin, a clone of rol A‐transformed tobacco was prepared. Auxin sensitivity was measured by the auxin concentration which induced the highest stimulation of H + ‐ATPase‐mediated proton translocation on isolated plasma membrane vesicles. Both transformed and control plants exhibited identical auxin‐sensitivity changes during vegetative and induction to flowering periods. However the sensitivity of flowering‐transformed plants Was 100‐times higher than that of control plants. Consistent observations were also made when using rol A+B+C‐transformed plants. These results suggest that the rol A gene product could either participate in or affect the reception/transduction pathway of auxin signal at the plasma membrane.