Premium
Autocrine receptors for endothelins in the primary culture of endothelial cells of human umbilical vein
Author(s) -
Fujitani Yasushi,
Oda Kyoko,
Takimoto Misato,
Inui Takashi,
Okada Toshikazu,
Urade Yoshihiro
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80026-d
Subject(s) - phosphoramidon , endothelins , umbilical vein , autocrine signalling , receptor , endothelin receptor , microbiology and biotechnology , chemistry , biology , biochemistry , in vitro
Human umbilical vein endothelial cells (HUVECs) in primary culture produced and secreted endothelin I (ET‐1) actively. Specific binding of [ 125 I]ET‐1 to these cells was not detectable because of the saturation of ET receptors with endogenously produced ET‐1. However, addition of phosphoramidon, an inhibitor of ET‐converting enzyme, to the medium reduced the production of ET‐1 and thus the receptors on HUVECs were made available for exogenously added [ 125 I]ET‐1. Binding studies using phosphoramidon‐treated HUVECs indicated the existence of a non‐isopeptide‐selective type (ET B ) or ET receptor with a K d of 17 pM. This receptor is thought to be involved in ET‐induced vasodilation in autocrine manner in vivo.