Premium
Characterization and differential expression of protein kinase C isoforms in PC12 cells Differentiation parallels an increase in PKC beta 11
Author(s) -
Wooten Marie W.,
Seibenhener M.Lamar,
Soh Yunjo,
Ewald Sandra J.,
White Kimberly R.,
Lloyd Elizabeth D.,
Olivier Andree,
Parker Peter J.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80025-c
Subject(s) - protein kinase c , gene isoform , neurite , pkc alpha , western blot , microbiology and biotechnology , nerve growth factor , biology , cytosol , kinase , chemistry , biochemistry , in vitro , receptor , enzyme , gene
Nerve growth factor (NGF) treatment of PC12 cells induced a 2.8‐fold increase in protein kinase C activity concomitant with differentiation and acquisition or neurites. PKC protein isoforms were separated by sequential chromatography on DEAE‐Sephacel/hydroxylapatite. A broad peak or PKC activity eluted which corresponded to the alpha PKC isoform. In control cells, message for all six PKC isoforms was detected and expressed as epsilon>zeta=gamma>delta>beta>alpha. Western blot or whole cell lysates revealed a large increase in the beta 11 , while slight changes were observed for the other five PKC isoforms during treatment (12‐14 days) with NGF (50 ng/ml). In parallel, coordinate changes in the expression of the individual transcripts for the six isoforms occurred during NGF treatment. Induction and accumulation of PKC beta 11 may play a role in maintenance or neuronal morphology.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom