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Production of endothelin‐1 and big‐endothelin‐1 by pleural mesothelial cells
Author(s) -
Kuwahara Masayoshi,
Kuwahara Maki,
Suzuki Nobuhiro
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80013-7
Subject(s) - mesothelial cell , endothelin receptor , endothelin 1 , chemistry , medicine , pathology , receptor
Immunoreactive endothelin‐1 (ET‐1) and big‐endothelin‐1 (big‐ET‐1) were detected in conditioned medium of cultured rat pleural mesothelial cells by using sensitive sandwich‐type enzyme immunoassays. The amount of both ET‐1 and big‐ET‐1 increased time‐dependently. In both instances, maximal amount was detectable in conditioned medium obtained after 72 h in culture (ET‐1: 117.1 ± 30.1 pg/10 6 cells; big‐ET‐1: 2.4 ± 2.1 pg/10 6 cells). Fetal calf serum markedly stimulated the production of both ET‐1 and big‐ET‐1.