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The sexual inducer of Volvox carteri
Author(s) -
Haas Elke,
Sumper Manfied
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81449-i
Subject(s) - yeast , inducer , saccharomyces cerevisiae , biochemistry , biology , gene
The DNA sequence coding for the sexual inducer glycoprotein of Volvox carteri and its N‐terminal signal peptide was placed under the control of the repressible acid phosphatase promoter of the yeast Saccharomyces cerevisiae in a yeast‐ E. coli shuttle vector. Yeast transformed by this construct synthesized and secreted into the culture medium biologically active inducer in amounts two to three orders of magnitude higher than observed in the Volvox system.