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Rapid turnover of low density lipoprotein receptor in human monocytic THP‐1 cells
Author(s) -
Hamanaka Ryoji,
Seguchi Tadashi,
Sato Yasufumi,
Ono Mayumi,
Kohno Kimitoshi,
Kuwano Michihiko
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81443-c
Subject(s) - thp1 cell line , monocytic leukemia , acute monocytic leukemia , receptor , monocyte , cell culture , low density lipoprotein , ldl receptor , chemistry , phorbol , lipoprotein , microbiology and biotechnology , biochemistry , biology , protein kinase c , signal transduction , cholesterol , immunology , genetics
We examined whether human monocyte‐derived macrophages had low density lipoprotein (LDL) receptors with a short life span. The human monocytic leukemia cell line, THP‐1, was highly differentiated when treated with phorbol ester. LDL receptors degraded rapidly with half‐lives of 3–4 h in THP‐1 cells before phorbol ester treatment. During the transition into monocytic cells, expression of the LDL receptor gene was not affected. However, relative degradation rates of LDL receptors normalized by those of cellular total proteins were about twice as fast in phorbol ester‐treated THP‐1 cells compared to untreated cells.