Premium
Evidence for a Competitive‐Displacement Model for the initiation of protein synthesis involving the intermolecular hybridization of 5 S rRNA, 18 S rRNA and mRNA
Author(s) -
Sarge Kevin D.,
Maxwell E.Stuart
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81437-d
Subject(s) - ribosomal rna , 23s ribosomal rna , messenger rna , biology , oligonucleotide , translation (biology) , genetics , microbiology and biotechnology , rna , ribosome , dna , gene
We have previously shown that a 5′‐terminal region of mouse 5 S rRNA can base‐pair in vitro with two distinct regions of 18 S rRNA. Further analysis reveals that these 5 S rRNA‐complementary sequences in 18 S rRNA also exhibit complementarity to the Kozak consensus sequence surrounding the mRNA translational start site. To test the possibility that these 2 regions in 18 S rRNA may be involved in mRNA binding and translational initiation, we have tested, using an in vitro translation system, the effects of DNA oligonucleotides complementary to these 18 S rRNA sequences on protein synthesis. Results show that an oligonucleotide complementary to one 18 S rRNA region does inhibit translation at the step of initiation. We propose a Competitive‐Displacement Model for the initiation of translation involving the intermolecular base‐pairing of 5 S rRNA 18 S rRNA and mRNA.