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Xylose‐specific antibodies as markers of subcompartmentation of terminal glycosylation in the Golgi apparatus of sycamore cells
Author(s) -
Lainé Anne-Catherine,
Gomord Véronique,
Faye Loïc
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81413-3
Subject(s) - golgi apparatus , glycosylation , xylose , chemistry , antibody , microbiology and biotechnology , biochemistry , specific antibody , brefeldin a , terminal (telecommunication) , biology , endoplasmic reticulum , immunology , fermentation , telecommunications , computer science
Antibodies specific for xylose‐containing plant complex N ‐linked glycans are used for indirect immunolocalization of xylosyltransferase in sycamore cells. The use of high pressure freezing and freeze substitution for sample preparation resulted in very good morphological preservation of the different Golgi cisternae. Xylosyltransferase shows a diffuse distribution all over the Golgi stacks and xylosylation appears to be an early processing event that is initiated in the cis Golgi compartment.