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Alternatively spliced mRNA of the pX region of human T lymphotropic virus type I proviral genome
Author(s) -
Furukawa Keiko,
Furukawa Koichi,
Shiku Hiroshi
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81404-v
Subject(s) - rna splicing , messenger rna , complementary dna , human t lymphotropic virus , microbiology and biotechnology , alternative splicing , biology , coding region , virus , virology , rna , gene , genetics , neuroscience , myelopathy , spinal cord
The pX region of human T lymphotropic virus type I (HTLV‐1) is believed to be expressed as a consequence of a 2 step splicing. It is conceivable, however, that a donor site of the 1st splicing and an acceptor site of the 2nd splicing results in the production of an alternatively spliced mRNA which is capable of coding p21 X‐III . This possibility was examined by amplifying cDNA derived from HTLV‐I + cells between the 5′ LTR and pX region. Bands of 2 different sizes were consistently observed. Sequencing of the longer band corresponded to a cDNA derived from a double‐spliced pX mRNA as previously reported. The shorter band was derived from a single‐spliced mRNA. HTLV‐I + cell lines had both mRNAs to a varying degree. Expression of p40 tax and p21 X‐III seem to be well correlated with a double‐spliced and a single‐spliced mRNA, respectively.