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Two isoforms of the muscle acetylcholine receptor α‐subunit are translated in the human cell line TE671
Author(s) -
Morris A.,
Beeson D.,
Jacobson L.,
Baggi F.,
Vincent A.,
Newsom-Davis J.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81399-s
Subject(s) - gene isoform , protein subunit , acetylcholine receptor , immunoprecipitation , alternative splicing , microbiology and biotechnology , reticulocyte , messenger rna , biology , scn3a , interleukin 10 receptor, alpha subunit , biochemistry , chemistry , receptor , g alpha subunit , gene
We have previously reported the existence of 2 forms of mRNA for the human muscle acetylcholine receptor (AChR) α‐subunit, thought to be generated by alternate splicing of a primary transcript and to encode 2 α‐subunit protein isoforms [1]. The 2 predicted α‐subunit isoforms, differing by the insertion of 25 amino acids at position , have been synthesized from cRNA transcripts using rabbit reticulocyte lysates: these protein isoforms could be differentiated by immunoprecipitation using antibodies raised against synthetic peptides. The antibodies were used to demonstrate translation of both AChR α‐subunit isoforms in the rhabdomyosarcoma (muscle) cell line TE671, in an approximate 1:1 ratio.