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Gene expression of type I phospholipase A 2 in pancreatic beta cells
Author(s) -
Metz S.,
Holmes D.,
Robertson R.P.,
Leitner W.,
Draznin B.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81397-q
Subject(s) - medicine , endocrinology , islet , hamster , pancreatic islets , messenger rna , phospholipase a2 , biology , gene expression , beta cell , phospholipase , beta (programming language) , insulin , phospholipase c , complementary dna , gene , chemistry , enzyme , biochemistry , programming language , computer science
Messenger RNA from intact rat pancreatic islets, or from transformed hamster beta (HIT) cells, hybridized with the cDNA probe for type I (but not type II) phospholipase A 2 . The levels of phospholipase A 2 mRNA increased in islets from fasted rats: they decreased in islets cultured in a high glucose concentration (control values at 5.5 mM glucose = 150±6% of those at 22 mM) which impaired subsequent insulin secretion (reduction in second‐phase release = 70±11%). These studies uniquely demonstrate that type I phospholipase A 2 is expressed specifically in beta cells and that nutrient availability modulates transcript levels, an effect which could contribute to the detrimental influence of prolonged hyperglycemia on islet function.