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Effects of the membrane potential upon the Ca 2+ ‐ and cumene hydroperoxide‐induced permeabilization of the inner mitochondrial membrane
Author(s) -
Novgorodov Sergey A.,
Gudz Tatjana I.,
Kushnareva Yulia E.,
Eriksson Ove,
Leikin Yury N.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81389-p
Subject(s) - protonophore , cumene hydroperoxide , membrane potential , biophysics , inner mitochondrial membrane , chemistry , permeability (electromagnetism) , mitochondrial permeability transition pore , mitochondrion , ruthenium red , membrane permeability , membrane , intracellular , biochemistry , biology , calcium , programmed cell death , apoptosis , organic chemistry , catalysis
A protonophore‐induced ΔΨ decrease in a 180‐140 mV range causes an increase in the lag‐period of Ca 2+ ‐induced mitochondrial permeabilization but has little effect on the cumene hydroperoxide‐induced permeability transition of mitochondria. Suppression of the non‐specific permeability induction seems to be mediated by an increase in [ADP] in the mitochondrial matrix. A further decrease in ΔΨ leads to additional suppression of the non‐specific permeability as a result of a partial ruthenium red‐sensitive efflux of the previously accumulated Ca 2+ . On the other hand, complete dissipation of ΔΨ causes immediate induction of the non‐specific permeability. It is concluded that only complete dissipation of ΔΨ caused by H + leakages may act as a trigger for non‐specific permeability induction.