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A C‐terminal proline is required for bioluminescence of the Ca 2+ ‐binding photoprotein, aequorin
Author(s) -
Nomura Midori,
Inouye Satoshi,
Ohmiya Yoshihiro,
Tsuji Frederick I.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81385-l
Subject(s) - photoprotein , aequorin , bioluminescence , proline , mutant , chemistry , biochemistry , biophysics , biology , amino acid , intracellular , gene
The requirement for a proline residue at the C‐terminus of the Ca 2+ ‐binding photoprotein, aequorin, was investigated by measuring luminescence activities of a series of C‐terminal deletion mutants, substitution mutants and an addition mutant. CD spectral measurements of apoaequorin with the C‐terminal proline deleted showed a small change in secondary structure. In all cases studied, the C‐terminal proline was required for bioluminescence activity.