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Tomato alcohol dehydrogenase
Author(s) -
Van Der Straeten Dominique,
Rodrigues Pousada Renato A.,
Gielen Jan,
Van Montagu Marc
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81379-m
Subject(s) - ripening , alcohol dehydrogenase , complementary dna , biology , messenger rna , blot , northern blot , solanaceae , enzyme , dehydrogenase , gene , gene expression , biochemistry , microbiology and biotechnology , botany
We have isolated and sequenced a partial tomato alcohol dehydrogenase ( Adh ) cDNA clone. Expression of tomato Adh was studied at the messenger RNA level in seedlings, roots, and fruit. High induction was observed under hypoxic conditions, both in tomato seedlings and in roots. In addition, the Adh mRNA was present at the mature green and pink stage of the tomato fruit, and was highly induced in late ripening. Moreover, an artificial ripening treatment resulted in at least 50‐fold induction compared to the mature green mRNA level. Genomic DNA gel blotting suggested the presence of a multigene family for Adh in tomato.