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Compositional properties of telomeric regions from human chromosomes
Author(s) -
De Sario Albertina,
Aïssani Brahim,
Bernardi Giorgio
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81375-i
Subject(s) - metaphase , telomere , biology , gene , genetics , microbiology and biotechnology , dna , human genome , genome , chromosome
We have investigated the GC levels of third codon position of genes localized in G‐ (Giemsa), R‐ (reverse) and T‐ (telomeric) bands of human metaphase chromosomes, as well as the hybridization of telomeric probes on fractionated human DNA. The first set of results shows much higher GC levels for genes localized in T‐bands than in G‐ or R‐bands (the latter being higher than the former). The second set of data shows that telomeric probes corresponding to T‐bands hybridize on the GC‐richest family (H3) of isochores, whereas telomeric probes corresponding to R‐bands hybridize on GC‐rich families H1 and H2; in agreement with these findings, the telomeric repeat common to all chromosomes hybridized on isochore families H1, H2 and H3.