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An hsp70 homolog is encoded on the plastid genome of the red alga, Porphyra umbilicalis
Author(s) -
Reith Michael,
Munholland Janet
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81355-c
Subject(s) - porphyra , plastid , biology , genome , red tide , genetics , algae , botany , gene , ecology , chloroplast
A PCR experiment using Porphyra umbilicalis DNA as the template and degenerate oligonucleotides representing conserved regions of hsp70 amino acid sequences generated a 1 kb product that hybridized exclusively to the plastid DNA of this red alga. DNA sequencing of two continguous Eco RI plastid DNA clones revealed a 620 amino acid open reading frame with 71% identity to the dnaK gene of the cyanobacterium. Synechocystis 6803. Northern hybridization experiments detected a 2.3 kb transcript that is present in control (15°C) cultures and increases approximately 7‐fold upon heat shock (75 minutes at 30°C).