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Demonstration of L1‐related mRNAs in rat brain using DNA oligonucleotide probes
Author(s) -
Gaardsvoll Henrik,
Andersson Anna-Maria,
Bock Elisabeth
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81350-h
Subject(s) - microbiology and biotechnology , messenger rna , oligonucleotide , complementary dna , biology , gene , dna , coding region , gene expression , genome , genetics
Only one copy of the cell adhesion molecule L1 gene is present in the mouse genome, and only one mRNA of 6 kilobases (kb) is expressed in mouse brain [1987, Neurosci. Lett. 82, 89–94]. We have constructed 5 synthetic oligonucleotide probes covering different parts of the published mouse L1 cDNA sequence. Using these probes 3 distinct mRNAs of 9.0, 7.0 and 6.0 kb in rat brain could be demonstrated. Hybridizations performed at different stringency conditions indicated that the 9.0 and 7.0 kb mRNAs were highly related to the L1 mRNA of 6.0 kb expressed in rat brain. The 7.0 kb mRNA is possibly coding for a rat homologue of chicken Nr‐CAM, whereas the 9.0 kb mRNA may code for a new member of the L1 family.