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Induction of neurite outgrowth of PC12 cells by an inhibitor of vacuolar H + ‐ATPase, bafilomycin A 1
Author(s) -
Tamura Hiro-omi,
Ohkuma Shoji
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81341-5
Subject(s) - bafilomycin , cycloheximide , neurite , nerve growth factor , microbiology and biotechnology , v atpase , atpase , phosphorylation , cell culture , chemistry , biology , biochemistry , in vitro , protein biosynthesis , apoptosis , enzyme , receptor , autophagy , genetics
Bafilomycin A 1 , a selective inhibitor of vacuolar H + ‐ATPase, induced neurite outgrowth of PC12 cells dose‐ and time‐dependently: more than 50% of the cells extended neurite‐like spikes after 24 h treatment with 100 nM bafilomycin A 1 . Its dose‐response ran roughly parallel to that of a bafilomycin A 1 ‐induced lysosomal pH increase. It was inhibited by LiCl, an inhibitor of the phosphorylation of microtubule‐associated proteins and, like nerve growth factor (NGF)‐induced neurite outgrowth, it was also inhibited by cycloheximide and actinomycin D. But, unlike the NGF‐effect, it was not associated with rapid induction of c‐fos .