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In vivo study of the state of order of the membranes of Gram‐negative bacteria by Fourier‐transform infrared spectroscopy (FT‐IR)
Author(s) -
Schultz Christian,
Naumann Dieter
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81339-a
Subject(s) - fourier transform infrared spectroscopy , fourier transform , membrane , in vivo , chemistry , infrared , spectroscopy , fourier transform spectroscopy , infrared spectroscopy , bacteria , gram , analytical chemistry (journal) , materials science , biochemistry , biology , physics , optics , chromatography , organic chemistry , genetics , microbiology and biotechnology , quantum mechanics
Temperature‐induced order/disorder transition profiles were obtained from the membranes of intact Gram‐negative bacterial cells by FT‐IR analysis of the frequency shifts of the acyl chain methylene symmetric stretching band as a monitor. Cells grown at different temperatures yielded distinct transition profiles. At the individual growth temperatures, however, the nearly alike frequency values indicated a very similar ‘state of order’ of the bacterial membranes. The FT‐IR data were complemented by GC analysis of whole cell fatty acid composition. The FT‐IR data obtained in vivo gave direct evidence of the adaptation of the ‘state of order’ and ‘fluidity’ of bacterial membranes to varying growth temperatures.