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Turnover of the K + transport system in Saccharomyces cerevisiae
Author(s) -
Benito Begoña,
Riballo Enriqueta,
Lagunas Rosario
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81337-8
Subject(s) - cycloheximide , saccharomyces cerevisiae , kinetics , yeast , transport system , chemistry , biochemistry , saccharomyces , substrate (aquarium) , enzyme kinetics , biophysics , protein biosynthesis , enzyme , biology , active site , physics , ecology , quantum mechanics , transport engineering , engineering
The stability of the K + transport system in Saccharomyces cerevisiae has been studied upon inhibition of protein synthesis with cycloheximide. Addition of the antibiotic gave rise to an inactivation of this transport. This activation followed first‐order kinetics and was stimulated by the presence of a fermentable substrate. A half‐life of about 4 h could be calculated in the presence of glucose. The results indicate that, similarly to sugar carriers, K + transport system is less stable than the bulk of proteins of this organism.