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PDGF suppresses the activation of group II phospholipase A 2 gene expression by interleukin I and forskolin in mesangial cells
Author(s) -
Mühl Heiko,
Geiger Thomas,
Pignat Werner,
Märki Fritz,
van den Bosch Henk,
Vosbeck Klaus,
Pfeilschifter Josef
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81295-j
Subject(s) - forskolin , platelet derived growth factor receptor , mesangial cell , medicine , genistein , tyrosine phosphorylation , endocrinology , phosphorylation , microbiology and biotechnology , tyrosine kinase , biology , tyrosine , secretion , gene expression , phospholipase a2 , chemistry , signal transduction , growth factor , gene , biochemistry , receptor , enzyme , stimulation , kidney
Treatment of rat mesangial cells with interleukin 1β (IL‐1β) and forskolin greatly enhanced the expression of group II phospholipase A 2 (PLA 2 ) mRNA, with subsequent increased synthesis and secretion of PLA 2 as detected by PLA 2 activity measurements and immunoprecipitation of culture media of [ 35 S]methionine‐labelled mesangial cells. PDGF—BB dose‐dependently suppressed the IL‐1β‐ and forskolin‐induced elevation of PLA 2 mRNA, as well as PLA 2 synthesis and secretion. In contrast, PDGF—AA had no inhibitory effect. The tyrosine kinase inhibitor genistein dose‐dependently antagonized the inhibitory effect of PDGF‐BB on IL‐1β‐stimulated PLA 2 secretion, thus suggesting that tyrosine phosphorylation may be required for PDGF‐BB inhibition of PLA 2 gene expression in mesangial cells.