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Molecular cloning and sequence analysis of cDNA and genomic DNA for the human cone transducin α subunit
Author(s) -
Kubo Mitsumasa,
Hirano Teiichi,
Kakinuma Mitsuaki
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81294-i
Subject(s) - microbiology and biotechnology , complementary dna , biology , protein subunit , jurkat cells , transducin , scn3a , genomic dna , gene , genetics , g protein , g alpha subunit , t cell , receptor , immune system
A novel GTP binding protein (G protein) α subunit cDNA was isolated from a T cell leukemia cell line, Jurkat, utilizing polymerase chain reaction (PCR). The predicted amino acid sequence of this G protein α subunit showed the highest identity (96.6%) to bovine cone cell‐specific transducin (T c α). The organization of the coding region of this G protein α subunit gene was composed of 8 exons and 7 introns. Northern hybridization revealed the presence of this G protein message in a retinoblastoma cell line, Y79. In Jurkat, however, the message was detectable only by reverse transcription/PCR. Taken together, this novel G protein α subunit must be human T c .