Premium
Co‐amplification and over‐expression of two mdr genes in a multidrug‐resistant human colon carcinoma cell line
Author(s) -
Chao Chuck C.-K.,
Ma Chang M.,
Lin-Chao Sue
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81287-i
Subject(s) - multiple drug resistance , gene , cell culture , cancer research , carcinoma , biology , microbiology and biotechnology , genetics , drug resistance
The human P‐glycoprotein gene family contains the mdr 1 and the mdr 3 gene. The mdr 1 P‐glycoprotein is over‐expressed in multidrug resistant (MDR) tumor cells and is believed to play a role in the elimination of certain cytotoxic drugs used in the chemotherapy of cancer. The mdr 3 gene has not been found to be amplified or over‐expressed in MDR cells. In this study, gene‐specific mdr gene probes were developed for the detection of the gene and the total mRNA level, Southern and Northern hybridization analyses showed that the mdr genes and the mRNA levels were increased 30–40‐fold in a MDR human colon cancer cell line. In addition, this MDR cell line had an altered growth rate and morphology and detectable double minute chromosomes.