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Cloning of segment polarity gene homologues from the unsegmented brachiopod Terebratulina retusa (Linnaeus)
Author(s) -
Holland Peter W.H.,
Williams Nicola A.,
Lanfear Jeremy
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81286-h
Subject(s) - biology , engrailed , gene , clone (java method) , genetics , cloning (programming) , gene duplication , genome , homeobox , gene expression , computer science , programming language
We have used the polymerase chain reaction (PCR) to amplify, clone and sequence homologues of the Drosophila segment polarity genes engrailed ( en ), cubitus interruptus Dominant ( ci D ) and wingless ( wg ) from the genome of the brachiopod, Terebratulina retusa (Linnaeus). The deduced translation products of brachiopod en and ci D share high levels of sequence identity with their Drosophila homologues. The brachiopod wg ‐related clone is divergent from Drosophila wg , although clearly a member of the wg/Wnt gene family. These results indicate that structural diversity of Drosophila segment polarity genes has been evolutionarily conserved in a divergent, ancient and unsegmented animal phylum.