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Functional expression of a human C5a receptor clone in Xenopus oocytes requires additional RNA
Author(s) -
Kroll Beate,
Emde Monica,
Jeromin Andreas,
Penner Lothar,
Rechkemmer Gerhard,
Kretzschmar Titus,
Klos Andreas,
Köhl Jörg,
Bautsch Wilfried
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81285-g
Subject(s) - xenopus , rna , in vitro , microbiology and biotechnology , biology , clone (java method) , transcription factor , chemistry , genetics , gene
cRNA from a PCR‐generated C5aR clone was prepared by in vitro transcription and microinjected into Xenopus laevis oocytes. Ligand‐induced whole cell current could be detected after co‐injection of cRNA for the C5aR with total RNA of the unstimulated U937 cell line, but not with either of the components injected alone. These data clearly demonstrate an absolute requirement of the C5aR for an additional human factor to become functionally expressed in Xenopus oocytes.