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Non‐cognizable ribonucleotide, 2′AMP, binds to a mutant ribonuclease T 1 (Y45W) at a new base‐binding site but not at the guanine‐recognition site
Author(s) -
Hakoshima Toshio,
Itoh Takeshi,
Gohda Keigo,
Tomita Ken-ichi,
Uesugi Sei-ichi,
Nishikawa Satoshi,
Morioka Hiroshi,
Ohtsuka Eiko,
Ikehara Morio
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81263-8
Subject(s) - guanine , ribonucleotide , binding site , ribonuclease t1 , chemistry , glycosidic bond , nucleotide , stereochemistry , hydrogen bond , active site , mutant , enzyme , biochemistry , molecule , rna , rnase p , organic chemistry , gene
Complex of a mutant ribonuclease T 1 (Y4SW) with a non‐cognizable ribonucleotide, 2′AMP, has been determined and refined by X‐ray diffraction at 1.7 Å resolution. The 2′AMP molecule locates at a new base‐binding site which is remote from the guanine‐recognition site, where 2′GMP was found to be bound. The nucleotide adopts the anti conformation of the glycosidic bond and C3′‐ exo sugar pucker. There exists a single hydrogen bond between the adenine base and the enzyme, and, therefore, the site found is apparently a non‐specific binding site. The results indicate that the binding of 2′AMP to the guanine‐recognition site is weaker than that to the new binding site.