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Biosynthesis of ferulic acid esters of plant cell wall polysaccharides in endomembranes from parsley cells
Author(s) -
Meyer Knut,
Kohler Annegret,
Kauss Heinrich
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81261-6
Subject(s) - digitonin , ferulic acid , chemistry , biochemistry , divalent , polysaccharide , centrifugation , vesicle , biosynthesis , enzyme , chromatography , membrane , organic chemistry
A microsomal preparation from suspension‐cultured parsley cells is able to transfer ferulic acid from the respective CoA thioester to endogenous acceptors. The reaction is not enhanced by digitonin but stimulated by Mg 2+ , Ca 2+ and Co 2+ . Spermine can partly replace divalent ions. Solubility properties and degradation by polysaccharide hydrolases suggest that the products are polymeric cell wall carbohydrates. Sucrose density gradient centrifugation revealed that the most active vesicle fraction is distinct from plasma membranes but does also not peak with IDPase. It is suggested that a subfraction of the Golgi‐apparatus is the source of enzyme and acceptors.