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Effects of cholesterol‐ or 7‐ketocholesterol‐containing liposomes on colony‐forming ability of cultured cells
Author(s) -
Khokhlov Alexander N.,
Prokhorov Leonid Yu.,
Ivanov Aleksey S.,
Archakov Alexander I.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81252-4
Subject(s) - liposome , phosphatidylcholine , cholesterol , incubation , hamster , chinese hamster , chemistry , biochemistry , biology , microbiology and biotechnology , in vitro , phospholipid , membrane
Experiments with cultured Chinese hamster cells showed that incubation of the cells with (phosphatidylcholine + cholesterol + 7‐ketocholesterol)‐containing liposomes (4:3:1 by weight) during two hours led to a decrease in the colony‐forming ability of cells down to zero, while (phosphatidylcholine + cholesterol)‐containing liposomes (1:1 by weight) reduce this parameter by 90%. Furthermore, the cholesterol‐containing liposomes (without 7‐ketocholesterol) induce a decrease in the number of the maximal‐site colonies accompanied by the corresponding increase in the number of the middle‐size colonies.