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A cyclic‐AMP‐gated conductance in cochlear hair cells
Author(s) -
Kolesnikov Stanislav S.,
Rebrik Tatyana I.,
Zhainazarov Aslbek B.,
Tavartkiladze George A.,
Kalamkarov Grigorii R.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81251-3
Subject(s) - divalent , patch clamp , biophysics , conductance , hair cell , chemistry , cyclic nucleotide gated ion channel , ion channel , inner ear , cyclic nucleotide , nucleotide , biochemistry , anatomy , biology , physics , receptor , organic chemistry , gene , condensed matter physics
The patch clamp technique was used to record cAMP‐dependent currents of the guinea pig cochlear hair cell plasma membrane. Data obtained indicate that the channels passing this current are moderately selective for monovalent cations and are effectively blocked by L‐ cis ‐diltiazem and reversibly blocked by 1 mM Mg 2+ or Ca 2+ . The single‐channel unit conductance estimated in the absence of divalent cations is about 16 pS. The results demonstrate that cyclic nucleotide‐dependent channels of cochlear hair cells are virtually identical to the photoreceptor and olfactory ones.