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Analysis of the 5′ non‐coding region of rat liver S ‐adenosylmethionine synthetase mRNA and comparison of the M r deduced from the cDNA sequence and the purified enzyme
Author(s) -
Alvarez Luis,
Asunción Miryam,
Corrales Fernando,
Pajares Maria A.,
Mato José M.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81245-4
Subject(s) - complementary dna , microbiology and biotechnology , messenger rna , coding region , biology , primer (cosmetics) , primer extension , cdna library , gene , biochemistry , chemistry , organic chemistry
A 3 kb cDNA coding for rat liver S ‐adenosylmethionine (AdoMet) synthetase has been isolated. The M r of the protein has been unequivocally determined by cDNA sequencing and enzyme purification on a thiopropyl‐Sepharose column. The length of the mRNA 5′ non‐coding region has been defined by primer‐extension analysis. The rat liver cloned cDNA has been also used to detect S ‐adenosylmethionine synthetase mRNA in human liver.