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Apolipoprotein D transcription occurs specifically in nonproliferating quiescent and senescent fibroblast cultures
Author(s) -
Provost Pierre R.,
Marcel Yves L.,
Milne Ross W.,
Weech Philip K.,
Rassart Eric
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81244-3
Subject(s) - fibroblast , messenger rna , biology , cell culture , microbiology and biotechnology , apolipoprotein b , transcription (linguistics) , gene expression , receptor , gene , cholesterol , biochemistry , genetics , linguistics , philosophy
We studied apolipoprotein D (apoD) mRNA in primary cultures of human diploid fibroblasts (HDF). In early‐passage HDF no apoD mRNA was detected in replicating cells in sparse culture, but the gene was expressed in quiescent cells in confluent and in serum‐starved cultures. In contrast, late‐passage HDF expressed apoD mRNA in sparse culture, but the level increased after attainment of confluence. Thus fibroblasts, the common cell‐type expressing apoD mRNA in vivo, express this characteristic following growth‐arrest. The same pattern of activation was found in another fibroblast cell line deficient in apoB/E (LDL) receptors, excluding a role for cellular cholesterol delivery by the LDL‐receptor pathway controlling apoD expression