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‘Neuron‐specific’ protein gene product 9.5 (PGP 9.5) is also expressed in glioma cell lines and its expression depends on cellular growth state
Author(s) -
Giambanco Ileana,
Bianchi Roberta,
Ceccarelli Paolo,
Pula Grazia,
Sorci Guglielmo,
Antonioli Sabrina,
Bocchini Virginia,
Donato Rosario
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81242-z
Subject(s) - immunocytochemistry , biology , neuron , glioma , gene product , carcinogenesis , gliosis , cell culture , neuroglia , cell growth , microbiology and biotechnology , gene expression , plating efficiency , gene , central nervous system , cancer research , genetics , neuroscience , endocrinology
Protein gene product 9.5 (PGP 9.5), which in the normal nervous system is restricted to certain neurons, has been detected in two glioma cell lines, rat C6 and human GL15, by immunoblotting and immunocytochemistry. Its expression in these cells depends on the cellular growth state, being maximal between the first and second post‐plating day. Only a faint PGP 9.5 immunoreactivity can be observed in glioma cells after the eleventh post‐plating day, i.e. about one week after confluency has been reached. The present results suggest that PGP 9.5 in cultured glial cells is maximally expressed during the growth phase and that the protein could play a role during brain development in glial cells, in reactive gliosis, or in tumorigenesis of the glial lineage.