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Strong expression of foreign genes following direct injection into fish muscle
Author(s) -
Hansen Ekkehard,
Fernandes Kenneth,
Goldspink Geoffrey,
Butterworth Peter,
Umeda Patrick K.,
Chang Kin-Chow
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81229-2
Subject(s) - chloramphenicol acetyltransferase , gene , reporter gene , myosin , microbiology and biotechnology , in vivo , beta galactosidase , plasmid , gene expression , biology , acetyltransferase , genetics , acetylation
We report here for the first time direct injection of genes into fish muscle in vivo. Plasmids used contain either SV40 early promoter, rabbit β‐cardiac myosin heavy chain promoter, human MxA promoter of an artificial promoter, fused to a chloramphenicol acetyltransferase (CAT) or β‐galactosidase reporter gene. CAT assays revealed that most gene constructs were highly expressed. Histochemical analysis showed that β‐galactosidase was strongly expressed at the site of injection within muscle fibres. This method provides an excellent system for testing expression of gene constructs, including those of mammalian origin, in fish muscle in vivo and has the potential for fish vaccination.