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Formation and crystallization of Thermus thermophilus 70S ribosome/tRNA complexes
Author(s) -
Yusupova Gulnara,
Yusupov Marta,
Spirin Alexander,
Ebel Jean-Pierre,
Moras Dino,
Ehresmann Chantal,
Ehresmann Bernard
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81228-z
Subject(s) - thermus thermophilus , crystallization , ribosome , transfer rna , chemistry , biochemistry , biology , crystallography , escherichia coli , rna , organic chemistry , gene
70S ribosomes from Thermus thermophilus are able to form ternary complexes with N ‐AcPhe‐tRNA Phe from either Thermus thermophilus or Escherichia coli , in the presence of a short oligo(U) of six or nine uridines. A complex of N ‐AcPhe‐tRNA Phe /(U) 9 /70S ribosome from Th. thermophilus was crystallized under the same conditions used for the growth of crystals from isolated ribosomes (S.D. Trakhanov, et al., (1987) FEBS Lett. 220, 319–322).