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Detection of the lipid‐linked precursor oligosaccharide of N ‐linked protein glycosylation in Drosophila melanogaster
Author(s) -
Parker G.F.,
Williams P.J.,
Butters T.D.,
Roberts D.B.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81225-w
Subject(s) - glycosylation , drosophila melanogaster , oligosaccharide , glycan , glycolipid , mannose , biochemistry , chemistry , glycoprotein , biology , gene
The presence of a glycan of the same molecular size as the lipid linked precursor oligosaccharide (Glc 3 Man 9 GlcNAc 2 ) of the N ‐linked protein glycosylation pathway in mammalian cells has been detected in a glycolipid fraction of cultured Drosophila melanogaster cells. Oligosaccharide sequencing studies were consistent with the existence of a glucosylated high mannose containing structure, which may be the common precursor for N ‐linked protein glycosylation in insect cells.