Premium
Cleavage reaction of a synthetic oligoribonucleotide corresponding to the autocleavage site of a precursor RNA from bacteriophage T4
Author(s) -
Hosaka Hideo,
Ogawa Takashi,
Sakamoto Kensaku,
Yokoyama Sigeyuki,
Takaku Hiroshi
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81187-d
Subject(s) - chemistry , cleavage (geology) , rna , phosphate , escherichia coli , ionic bonding , bacteriophage , stereochemistry , biochemistry , organic chemistry , ion , biology , paleontology , fracture (geology) , gene
A fragment (GUUUCGUACAAAC) having a consensus sequence for the self‐cleavage domain in a precursor of an RNA molecule from T4‐infected Escherichia coli cells (p2Sp1; precursor of species 1) was chemically synthesized and found to be cleaved either between CA (139–140) or between UA (137–138) in the presence of monovalent cations and a non‐ionic detergent. The cleaved products had 5′‐hydroxyl and 3′‐phosphate groups, of which some were in the form 2′.3′‐cyclic phosphates.