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Inositol triphosphate produces different patterns of cytoplasmic Ca 2+ spiking depending on its concentration
Author(s) -
Petersen Carl C.H.,
Toescu Emil C.,
Potter Barry V.L.,
Petersen Ole H.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81181-7
Subject(s) - inositol , intracellular , cytosol , second messenger system , inositol phosphate , biophysics , inositol trisphosphate , chemistry , inositol trisphosphate receptor , patch clamp , receptor , biochemistry , biology , enzyme
In single mouse pancreatic acinar cells the effects of intracellular infusion of inositol 1,4,5‐trisphosphate (InsP 3 ) or the non‐metabolizable InsP 3 analogue inositol 1,4,5‐triphosphorothioate (InsPS 3 ) have been investigated using a wide range of concentrations. Different types of cytosolic Ca 2+ fluctuation patterns (monitored as Ca 2+ ‐dependent Cl − current in patch‐clamp whole‐cell recording experiments) could be generated by InsP 3 or InsPS 3 , dependent on concentration, resembling those previously shown to be evoked by varying degrees or receptor activation in these cells. Low InsPS 3 concentrations evoked repetitive local Ca 2+ spikes whereas at relatively high concentrations repetitive Ca 2+ waves were produced. In the presence of intracellular citrate a much lower messenger level was sufficient to generate waves. The InsP 3 concentration determines whether the cytosolic Ca 2+ signals are local or global.