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Repression of the CaMV 35S promoter by the octopine synthase enhancer element
Author(s) -
Gatz Christiane,
Katzek Jens,
Prat Salome,
Heyer Arnd
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81180-g
Subject(s) - octopine , enhancer , psychological repression , genetics , biology , chemistry , microbiology and biotechnology , gene , transcription factor , transformation (genetics) , agrobacterium , gene expression
A 16 base‐pair palindrome upstream of the Agrobacterium tumefaciens octopine synthase ( ocs ) gene functions as a positive regulatory element in plant cells (Ellis et al. (1987) EMBO J. 6, 3203–3208; Fromm et al. (1989) Plant Cell 1, 977–984). We have converted it into a negative element by locating two copies flanking the TATA‐box of the constitutively expressed CaMV 35S promoter. The reduced promoter activity is very likely due to sterical hindrance of the ocs binding protein (OCSTF) × ocs complex with the transcription initiation complex. We propose that this type of constructs can be used for the identification of recognition sites for DNA‐binding proteins which are labile in vitro as well as for determining the DNA‐binding activity of a trans ‐acting factor in vivo.